Effect of Frequency of Subculturing of different Isolates of Beauveria bassiana Vuillemin on their Biological Properties

Authors

  • P. Rajanikanth District Agricultural Advisory and Transfer of Technology Centre (DAATTC), H.No.6-3-253, Rudrama Nilayam, Ramagiri, Nalgonda, Andhra Pradesh (508001), India
  • G. V. Subbaratnam Department of Entomology, College of Agriculture, Acharya N.G.Ranga Agricultural University, Rajendranagar, Hyderabad, Andhra Pradesh (500 030), India
  • S. J. Rahaman Department of Entomology, College of Agriculture, Acharya N.G.Ranga Agricultural University, Rajendranagar, Hyderabad, Andhra Pradesh (500 030), India

Keywords:

Beauveria bassiana, subculturing, biologi­cal properties, molecular characterization

Abstract

An experiment was conducted to find the effect of repeated subculturing on biological properties viz., radial growth, sporulation and conidial viability of four strains (Bb-13, Bb-11, Bb-5A and Bb-N) and two local isolates (Bb-L-1 and Bb-L-2) of Beauveria bassiana Vuillemin on synthetic media. The results of the experiment showed no significant reduction of the biological properties during the initial 1st to 7th subculturing but showed significant negative effects on the biological properties after 7th subculturing. Molecular characterization of the strains using PCR based RAPD indicated considerable variability among the isolates.  

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Published

2023-04-21

How to Cite

1.
Rajanikanth P, Subbaratnam GV, Rahaman SJ. Effect of Frequency of Subculturing of different Isolates of Beauveria bassiana Vuillemin on their Biological Properties. IJBSM [Internet]. 2023 Apr. 21 [cited 2025 Sep. 7];2(Mar, 1):60-5. Available from: https://ojs.pphouse.org/index.php/IJBSM/article/view/107

Issue

Vol. 2 No. Mar, 1 (2011)

Section

Articles

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