Morphomolecular Identification of Trichoderma sp. and their Mycoparasitic Activity Against Soil Borne Pathogens

Authors

  • Gyanendra Kumar LPS Cardiology, Kanpur, Uttar Pradesh (208 019), India
  • Anuradha Singh CSAUA&T, Kanpur, Uttar Pradesh (208 002), India
  • Sonika Pandey ICAR-IIPR, Kanpur, Uttar Pradesh (208 024), India
  • Jogender Singh LPS Cardiology, Kanpur, Uttar Pradesh (208 019), India
  • Sangeeta Singh Chauhan LPS Cardiology, Kanpur, Uttar Pradesh (208 019), India
  • Mukesh Srivastava Rani Laxmi Bai Central Agriculture University, Jhansi, Uttar Pradesh (284 003), India

Keywords:

Morphology, genetic identification, trichokey, mycoparasitic activity

Abstract

This study was carried out to identify and characterize Trichoderma species isolated from rhizospheric soil of Uttar Pradesh, India, by using single spore technique. Morphological, cultural and molecular characterization were done with sequence analysis of the internal transcribed spacer (ITS) region. The classes were compared with morphological identification and rDNA sequence data for every class of all Trichoderma strains were of the same identity. These strains belonged to T. harzianum (Th azad), T. viride  (01PP), T. asperellum (Tasp/CSAU), T. Koningii [TK (CSAU)], T. atroviride (71L), T. longibrachiatum (21PP), T. virens [Tvi (CSAU)], T. reesei [Tr (CSAU)], T. aggressivum [T.agg(CSAU)], T. aureoviride [T. avi (CSAU)], T. citrinoviride [T. cvi (CSAU)],  T. erinaceum [T. eri (CSAU)], T. koningiopsis [T. kop (CSAU)], T. tomentosum [T. tos (CSAU)], T. mintisporum [T. mip (CSAU)], T. pubscenes [T. sce (CSAU)], T. saturnisporum [T. ssp (CSAU)], T. spirale [T. sp. (CSAU)]. Morphological studies were based on the colony appearance, growth rate and microscopic features such as branching patterns of conidiophores, the arrangement of phialospores and their shape, size and color. The 5.8S-ITS regions of the Trichoderma strains were amplified using ITS1 and ITS4 primers. The rRNA based analysis is a central method used not only to explore microbial diversity but also to identify new strains. Validations of ITS marker with 18 Trichoderma sp. were done and their sequences were deposited at NCBI GenBank their permanent accession no. were allotted.

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Published

2021-01-14

How to Cite

1.
Kumar G, Singh A, Pandey S, Singh J, Chauhan SS, Srivastava M. Morphomolecular Identification of Trichoderma sp. and their Mycoparasitic Activity Against Soil Borne Pathogens. IJBSM [Internet]. 2021 Jan. 14 [cited 2025 Sep. 10];11(Dec, 6):613-27. Available from: https://ojs.pphouse.org/index.php/IJBSM/article/view/4060

Issue

Vol. 11 No. Dec, 6 (2020)

Section

Articles

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