Genetic Diversity Assessment in Pea (Pisum sativum L.) using Microsatellite Markers

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Authors

  • Hanuman Ram Division of Crop Improvement, ICAR-Central Institute for Arid Horticulture, Bikaner, Rajasthan (334 006), India
  • Nirmal K. Hedau Crop Improvement Division, ICAR-Vivekananda Parvatiya Krishi Anusandhan Sansthan, Almora, Uttarakhand (263 601), India
  • Ganesh V. Chaudhari Horticulture Section, ICAR-Central Coastal Agricultural Research Institute, Old Goa (403 402), India
  • Mukesh Choudhary ICAR-Indian Institute of Maize Research, Ludhiana, Punjab (141 004), India
  • Lakshmi Kant Crop Improvement Division, ICAR-Vivekananda Parvatiya Krishi Anusandhan Sansthan, Almora, Uttarakhand (263 601), India

Keywords:

Peas, genetic diversity, SSR markers, PIC

Abstract

The present study was conducted on genetic diversity analyses among 24 pea genotypes during 2017–2018 to assess the molecular diversity of pea genotypes using SSR markers. Out of 62, eleven markers were found to be polymorphic and the polymorphic information content (PIC) of the simple sequence repeat (SSR) markers ranged from 0.19 to as high as 0.64. Molecular profiling of these genotypes using 11 SSRs distributed throughout the genome generated 32 alleles with a mean of 2.91 alleles per locus. The genetic dissimilarity based on simple matching coefficient for 24 genotypes ranged from 0.00 to 0.91 with an average of 0.52. Cluster analyses grouped 24 genotypes into two major clusters with one outlier and supported by principal coordinate analysis (PCoA) in which genotypes were distributed across four quadrangles. Analysis of molecular variance (AMOVA) showed significant estimated value at degree of 1000 permutations. Percentage of variability was higher among individual (67%) than among populations (11%). Percentage of variability within individual was also higher (22%) than among populations (11%). Pop1 (I=0.707, He=0.446, and uHe=0.466) shows higher diversity than pop2 (I=0.630, He=0.381 and uHe=0.398). The percentage of polymorphic loci per population (PPL) ranged from 81.82% (pop2) to 90.91% (pop1) with an average of 86.36%. The present study demonstrates the utility of microsatellite markers for estimating molecular diversity as well as genotype identification in pea. This study also suggests a potential use of these markers in further association studies.

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Published

2021-09-11

How to Cite

1.
Ram H, Hedau NK, Chaudhari GV, Choudhary M, Kant L. Genetic Diversity Assessment in Pea (Pisum sativum L.) using Microsatellite Markers. IJBSM [Internet]. 2021 Sep. 11 [cited 2024 Feb. 21];12(Aug, 4):402-8. Available from: https://ojs.pphouse.org/index.php/IJBSM/article/view/4122

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