Identification of Polymorphism within Exon 8 of Bovine HSP90AA1 Gene using PCR-SSCP Technique

Authors

  • J. Sai Prasanna College of Veterinary Science, P. V. Narsimha Rao Telangana Veterinary University, Rajendranagar, Hyderabad, Telangana State (500 030), India
  • S. T. Viroji Rao College of Veterinary Science, P. V. Narsimha Rao Telangana Veterinary University, Rajendranagar, Hyderabad, Telangana State (500 030), India
  • M. Gnana Prakash College of Veterinary Science, P. V. Narsimha Rao Telangana Veterinary University, Rajendranagar, Hyderabad, Telangana State (500 030), India
  • Suresh Rathod College of Veterinary Science, P. V. Narsimha Rao Telangana Veterinary University, Rajendranagar, Hyderabad, Telangana State (500 030), India
  • P. Kalyani College of Veterinary Science, P. V. Narsimha Rao Telangana Veterinary University, Rajendranagar, Hyderabad, Telangana State (500 030), India
  • B. Rajith Reddy College of Veterinary Science, P. V. Narsimha Rao Telangana Veterinary University, Rajendranagar, Hyderabad, Telangana State (500 030), India

Keywords:

Crossbred cows, Exon 8, Heat stress, HSP90AA1, Polymorphisms, Sahiwal

Abstract

Heat shock proteins (HSPs) are a type of molecular chaperones that aid in the recovery of stressed cells and serve as a major system for intracellular self-defense. A study was conducted during the year 2018–19 at College of Veterinary Science Rajendranagar, Hyderabad, Telengana State, India to find polymorphisms in exon 8 of the bovine HSP90AA1 gene in Sahiwal (n=50) and crossbred (n=50) cows. Blood samples were collected from the experimental animals and genomic DNA was isolated. Physiological parameters like body temperature and respiration rate for each animal were taken during the experimental period and the heat tolerance coefficient was calculated. The data on production and reproduction traits were obtained from the history sheets of the animals. To detect the polymorphism, a 539 bp fragment of the HSP90AA1 gene covering exon 8 was subjected to the Polymerase Chain Reaction-Single-Strand Conformation Polymorphism (PCR-SSCP) technique.The PCR-SSCP of exon 8 of HSP90AA1 gene yielded two genotypic patterns AA and AB corresponding to two allelic variants with frequencies of 0.85, 0.15, 0.81 and 0.19 in Sahiwal and crossbred cows, respectively. The PCR-SSCP patterns obtained were correlated with the physiological, productive, and reproductive traits in both Sahiwal and crossbred cows. The association analysis of SSCP patterns of the exon 8 of HSP90AA1 gene revealed non-significant effect in Sahiwal cows, although the AB genotype had a significantly longer service period in crossbred cows.

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Published

2021-12-10

How to Cite

1.
Prasanna JS, Rao STV, Prakash MG, Rathod S, Kalyani P, Reddy BR. Identification of Polymorphism within Exon 8 of Bovine HSP90AA1 Gene using PCR-SSCP Technique. IJBSM [Internet]. 2021 Dec. 10 [cited 2024 May 25];12(Dec, 6):611-6. Available from: https://ojs.pphouse.org/index.php/IJBSM/article/view/4151

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