Evaluation of Cultivated and Wild Brinjal Germplasm against Bacterial Wilt Disease with Tollinterleukin-1 Receptors (TIR)-NBS-LRR Type R-gene Specific Degenerate Primer

Authors

  • S. Hansda Dept. of Plant Pathology, Faculty of Agriculture, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal (741 246), India.
  • I. Jamir Dept. of Plant Pathology, Faculty of Agriculture, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal (741 246), India.
  • K. Pramanik Dept. of Agricultural Biotechnology, Faculty of Agriculture, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal (741 246), India.
  • J. Banerjee Dept. of Agricultural and Food Engineering, Indian Institute of Technology, Kharagpur, West Bengal (721 302), India
  • A. Chattopadhyay Dept. of Vegetable Science, Faculty of Horticulture, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal (741 246), India.
  • A. K. Mandal Dept. of Plant Pathology, Faculty of Agriculture, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal (741 246), India.

Keywords:

Bacterial wilt (BW), brinjal, resistant genotype, screening wilt resistant

Abstract

The experiment was conducted at C-Block Farm of  Bidhan Chandra Krishi Viswavidyalaya,  Kalyani, West Bengal, India during 2017–18 to screen eight brinjal germplasm lines against BW disease using tollinterleukin-1 receptors (TIR)-NBS-LRR type R-gene specific degenerate primer. The study showed that wild genotype S. torvum was highly resistant to bacterial wilt incidence with no wilting symptom whereas two cultivated genotypes (Utkal Anushree and Utkal Madhuri) and one wild genotype S. sisymbriifolium were found to be resistant to BW disease. Out of the 7 germplasm sequences, 2 had no match with R-genes whereas the remaining 5 sequences have 70-93% homology with R-genes of other plant species submitted in Gene Bank sequence database. Nearly 90% sequence identity of brinjal NBS-LRR RGA was found by analyzing through BLASTn with NBS-LRR RGAs of other solanaceous crops. Two cultivated resistant genotypes (Utkal Madhuri and Utkal Tarini) were similar to the wild resistant type S. sisymbriifloium, while cultivable resistant genotype Utkal Anushree was highly different at sequence level. Two cultivable susceptible genotypes (BCB-30 and Garia) showed high level of similarity among them and they were strongly associated with the wild susceptible genotype S. macrocarpum. Two cultivable genotypes Utkal Anushree and Utkal Madhuri could be utilized in future breeding programme and two wild genotypes S. torvum and  S. sisymbriifolium could be used as resistant rootstocks in brinjal grafting.

Downloads

Download data is not yet available.

Downloads

Published

2021-12-31

How to Cite

1.
Hansda S, Jamir I, Pramanik K, Banerjee J, Chattopadhyay A, Mandal AK. Evaluation of Cultivated and Wild Brinjal Germplasm against Bacterial Wilt Disease with Tollinterleukin-1 Receptors (TIR)-NBS-LRR Type R-gene Specific Degenerate Primer. IJBSM [Internet]. 2021 Dec. 31 [cited 2024 May 25];12(Dec, 6):670-8. Available from: https://ojs.pphouse.org/index.php/IJBSM/article/view/4158

Issue

Section

Articles