Screening for In Vitro Antibacterial Activity of Leaves Extracts of Certain Selected Plants against Rhodococcus equi

Lalit Kumar; Laxmi Narayan Sankhala; Lakshmi Kant; Dinesh Kumar Badsiwal; Sanjay Kumar; Ramesh Kumar Dedar

doi:10.23910/1.2023.3257

Authors

Lalit Kumar Dept. of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Science, RAJUVAS, Bikaner, Rajasthan (334 001), India
Laxmi Narayan Sankhala Dept. of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Science, RAJUVAS, Bikaner, Rajasthan (334 001), India https://orcid.org/0000-0002-5340-4805
Lakshmi Kant Dept. of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Science, RAJUVAS, Bikaner, Rajasthan (334 001), India
Dinesh Kumar Badsiwal Dept. of Epidemiology and Preventive Veterinary Medicine, College of Veterinary and Animal Science, RAJUVAS, Bikaner, Rajasthan (334 001), India
Sanjay Kumar ICAR-NRCE, Hisar, Haryana (125 001), India
Ramesh Kumar Dedar ICAR-NRCE, Equine Production Campus, Bikaner, Rajasthan (334 001), India

DOI:

https://doi.org/10.23910/1.2023.3257

Keywords:

Antibacterial, Azadirachta indica, in vitro, leaves, Rhodococcus equi

Abstract

In the present study, the research work was carried out during June 2018 to March 2019 at ICAR-NRCE-EPC (Indian Council of Agricultural Research, National Research Centre on Equines, Equine Production Campus), Jorbeer, Bikaner (Rajasthan), India. The study was conducted to investigate in vitro antibacterial activity of ethanolic, chloroformic and Sequentially Extracted Water Extract (SEWE) leaves extracts of Aegle marmelos, Morus alba, Nerium indicum, Cascabela thevetia (L.) Lippold, Plumeria alba L. and Azadirachta indica against Vap A and Vap C positive Rhodococcus equi. Fresh leaves of these plants were collected manually from campus of ICAR-NRCE-EPC, Jorbeer, Bikaner (Rajasthan). In initial screening ethanolic leaves extract of these plants except Azadirachta indica, were found non-active against Rhodococcus equi. Chloroformic leaves extracts of Azadirachta indica did not showed in vitro antibacterial activity against Rhodococcus equi. While ethanolic and Sequentially Extracted Water Extract (SEWE) leaves extracts of Azadirachta indica showed good in vitro antibacterial activity against Rhodococcus equi. Further, solvent based fractionation, Ethanol Soluble Fraction (ESF), Methanol Soluble Fraction (MSF) and Water Soluble Fraction (WSF) of polar compounds of SEWE did not showed in vitro antibacterial activity against Rhodococcus equi. On comparison with currently used antibiotics (azithromycin and rifampicin), required concentration of the leaves extract of Azadirachta indica was too high for their possibilities of in vivo use, so abundant availability of Azadirachta indica leaves and their activity against Rhodococcus equi suggests their potential for use as disinfectant against Rhodococcus equi.