Effect of Epidermal Growth Factor on In Vitro Maturation, Fertilization and Early Embryonic Development of Cattle Oocytes

Authors

  • Dipannita Baishya Bovine Contagious Pleuro-Pneumonia, Khanapara, Guwahati, Assam (781 022), India
  • Arundhati Bora Dept. of Veterinary Physiology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam (781 022), India
  • Champak Barman Dept. of Veterinary Physiology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam (781 022), India https://orcid.org/0000-0001-9702-8680

DOI:

https://doi.org/10.23910/1.2024.5539

Keywords:

Cattle, cleavage, epidermal growth factor, oocyte

Abstract

The present experiment was conducted during January, 2018 to March, 2019 in the laboratory, Dept. of Veterinary Physiology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam (781022), India to investigate the effect of epidermal growth factor on in vitro maturation, fertilization and early embryonic development of cattle oocytes. A total of 318 nos. cattle ovaries were collected from slaughter houses for one year and 1089 nos. culturable oocytes were collected. 381 out of 1089 nos. culturable oocytes were subjected to EGF supplemented serum and serum free basic maturation media. The mean percentage of in-vitro maturation of cattle oocytes based on polar body extrusion was found to be significantly higher in EGF supplemented serum free basic maturation media (70.00±14.49) than the serum basic maturation media without EGF (54.17±7.19) respectively. But the mean percentages of in vitro maturation of cattle oocytes based on the cumulus expansion and polar body extrusion were found to be significantly higher in EGF supplemented serum free basic maturation media (77.59±5.48 and 70.00±14.49) than the value recorded in serum free basic maturation media without EGF (64.20±3.77 and 45.95±8.19). The mean cleavage percentages recorded at 4 cell, 8 cell, 16 cell and morula stages of embryos in EGF supplemented serum free culture media were found to be significantly higher than the value recorded in serum culture media without EGF. From the present experiment, it can be inferred that addition of EGF in serum free basic maturation media had little beneficial effect than serum basic maturation media.

Downloads

Download data is not yet available.

Downloads

Published

2024-09-04

How to Cite

1.
Baishya D, Bora A, Barman C. Effect of Epidermal Growth Factor on In Vitro Maturation, Fertilization and Early Embryonic Development of Cattle Oocytes. IJBSM [Internet]. 2024 Sep. 4 [cited 2025 Sep. 20];15(Sep, 9):01-9. Available from: https://ojs.pphouse.org/index.php/IJBSM/article/view/5539

Issue

Vol. 15 No. Sep, 9 (2024)

Section

Articles

License

Copyright (c) 2024 International Journal of Bio-resource and Stress Management

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Authors retain copyright. Articles published are made available as open access articles, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited. Creative Commons License

This journal permits and encourages authors to share their submitted versions (preprints), accepted versions (postprints) and/or published versions (publisher versions) freely under the CC BY-NC-SA 4.0 license while providing bibliographic details that credit, if applicable.