In-vitro Phosphate Solubilization by Native Phosphate Solubilizing Microbes in Soils of Northern Region of India

Akansha Singh; B. P. Dhyani; Akash Tomar; Vipin Kumar; Arbind Kumar Gupta; U. P. Shahi

doi:10.23910/1.2022.3274a

Authors

Akansha Singh Dept. of Soil Science and Agricultural Chemistry, Sardar Vallabhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh (250 110), India
B. P. Dhyani Dept. of Soil Science and Agricultural Chemistry, Sardar Vallabhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh (250 110), India
Akash Tomar Dept. of Recombinant Technology, Sardar Vallabhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh (250 110), India
Vipin Kumar Dept. of Soil Science and Agricultural Chemistry, Sardar Vallabhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh (250 110), India
Arbind Kumar Gupta Dept. of Natural Resource Management (Soil Science), Banda University of Agriculture & Technology, Banda, Uttar Pradesh (210 001), India https://orcid.org/0000-0002-9772-8078
U. P. Shahi Dept. of Soil Science and Agricultural Chemistry, Sardar Vallabhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh (250 110), India

DOI:

https://doi.org/10.23910/1.2022.3274a

Keywords:

Phosphate enrichment, microbial population, phosphorus solubilization, soil fertility

Abstract

The investigations were conducted at Sardar Vallabhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh, India during March 2019 to January 2020 for isolation and characterization and identification of phosphate solubilizing microbes (PSM) in soils of northern region of India. Soil samples (0-30 cm) were collected from four locations i.e. KVK, Lohaghat, SVPUAT, Meerut, College of Agriculture field, SVPUAT, Meerut and KVK, Bulandshahar covering Uttar Pradesh and Uttarakhand states of India and kept in refrigerator for analysis. The isolation of two rhizobial bacteria isolates (S₁ and S₂) in conjunction with the two-phosphate solubilizing efficient fungal isolates (F₁ and F₂) was carried out by the serial dilution method. The study of their morphological and microscopic characters, together with Solubilization Efficiency (SE), and Solubilization Index (SI) were also assessed. Finally, the biochemical tests were run according to their nature that was gram-negative rod by using KB002 TM HI Assorted Biochemical Test Kit (for Gram-negative rods). Between the two bacterial isolates was S₁ was showing more solubilization on Pikovskaya agar (PVK) compared to S₄ on the 5^th day of observation, while S₄ gave better results on the 8^th-day solubilization observation. The biochemical tests on bacterial isolates were showing disagreement between the two, proving them as completely different isolates. In the case of fungi, the F₂ isolate was showing better SE and SI values compared to the F₁ isolate.