Effect of Henna Leaves (Lawsonia inermis) Incorporated Fish Feed on Growth, Survival and Pigmentation of Guppy, Poecilia reticulata (Peters, 1859)

Authors

  • Imran Mohammed Dept. of Aquaculture, TNJFU-Dr. MGR Fisheries College and Research Institute, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, Thalainayeru, Tamil Nadu (614 712), India
  • Loganathan Kowsigan Dept. of Aquaculture, TNJFU-Dr. MGR Fisheries College and Research Institute, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, Thalainayeru, Tamil Nadu (614 712), India
  • Muthu Abishag Dept. of Aquaculture, TNJFU-Dr. MGR Fisheries College and Research Institute, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, Thalainayeru, Tamil Nadu (614 712), India https://orcid.org/0000-0003-3014-5393
  • Mohamed Faizullah Dept. of Aquaculture, TNJFU-Dr. MGR Fisheries College and Research Institute, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, Thalainayeru, Tamil Nadu (614 712), India
  • Mohamed Meeran Dept. of Aquaculture, TNJFU-Dr. MGR Fisheries College and Research Institute, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, Thalainayeru, Tamil Nadu (614 712), India
  • Ranjith Kumar Dept. of Fisheries Biology and Resource Management, TNJFU-Dr. MGR Fisheries College and Research Institute, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, Thalainayeru, Tamil Nadu (614 712), India
  • Pandi Kalaiselvan Dept. of Fish nutrition and Feed technology, TNJFU - Institute of Fisheries Post Graduate Studies, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, Vaniyanchavadi, Tamil Nadu (641 107), India

DOI:

https://doi.org/10.23910/1.2026.6955

Keywords:

Colouration, feed additives, fish diet, guppy, Lawsonia inermis

Abstract

The study was conducted during April, 2024–June, 2024 at Aquaculture wet laboratory of Dr. MGR Fisheries College and Research Institute, Thalainayeru. This research sought to examine how incorporating powdered dried henna leaves (Lawsonia inermis) into fish food impacts the growth, survival and pigmentation of guppy. For the experimental trial, 120 male guppies were stocked in 8 tanks (30 fishes treatment-1 in duplicate). The fish were fed with four isonitrogenous diets (33% Crude Protein) with different concentrations of Henna incorporation: Control (0%), H1 (1%), H2 (2%), and H3 (3%) for 60 days. At the end of the experiment, fish samples were analysed for growth parameters, survival, and pigment accumulation. Compared to the control group, H3 exhibited significantly higher (p<0.05) growth indicators, including specific growth rate, net weight gain, and weight gain percentage. The survival rate was significantly greatest (p<0.05) in H2, followed by H3, H1, with lowest survival rate observed in control group. The tissue carotenoid concentration was significantly (p<0.05) highest in control group, followed by H2, H3 and was significantly (p<0.05) lowest in H1. Colour intensity assessment using image J software® revealed that red colour exhibited significant lower value (p<0.05) in H3 compared to other groups (control, H1 and H2), while the green and blue colours exhibited significant greater value (p<0.05) in the control compared to all treatment groups (H1, H2 and H3). The present study demonstrated that incorporating 3% dried leaf powder of Lawsonia inermis in guppy feed enhanced growth parameters while simultaneously reducing carotenoid content. However, 2% incorporation was found to improve the survival rate.

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Published

2026-05-25

How to Cite

1.
Mohammed I, Kowsigan L, Abishag M, Faizullah M, Meeran M, Kumar R, et al. Effect of Henna Leaves (Lawsonia inermis) Incorporated Fish Feed on Growth, Survival and Pigmentation of Guppy, Poecilia reticulata (Peters, 1859). IJBSM [Internet]. 2026 May 25 [cited 2026 Jul. 18];17(May, 5):01-10. Available from: https://ojs.pphouse.org/index.php/IJBSM/article/view/6955

Issue

Vol. 17 No. May, 5 (2026)

Section

Articles

License

Copyright (c) 2026 Imran Mohammed, Loganathan Kowsigan, Muthu Abishag, Mohamed Faizullah, Mohamed Meeran, Ranjith Kumar, Pandi Kalaiselvan

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